Lactic dehydrogenase inhibitors in NAD.

The vacuum oven-drying method (60#{176}C) was compared with the freeze-drying method. By the former method, 72.85 ± 0.0374 (SD) % moisturewas removed, by thelatter, 81.04 ± 0.050%.The feces were from the same source. With the freeze-drying method total moisture removed is clearly greater, and the time required was only 16 h, as compared with 48 h required by the vacuum oven-drying method. There are other distinct advantages. Since measurement of the total energy of the sample is based on a rise in temperature at the point of combustion,freeze-drying duringpreparation eliminates any possibility of a rise in temperature that might lead to an error in calculating the total energy. Drying to a constant weight, which necessitates timeconsuming multipleweighings,is not required.The driedsample isintheform ofa powder and requiresno grinding. Unpleasant odors associated with the ovendrying method are avoided. To bettercompare the old oven-drying method and the new freeze-drying method, caloricontentof four specimens ofthesame sampleoffeceswas measured by each method. The results were in good agreement (Table 1). The results shown in Table 1 for the wet weight in kilogram-calories per gram are calculated by multiplying the dry weight (kcal/g) by the percent remaining moisture (100% minus the percent moisture removed). We feel that this method represents a distinct improvement over the original methods.